Relative response factor of impurity = Calculation of Relative Response Factor (RRF) in Gas Chromatography where, the internal standard used. Concentration A = (Peak area A / Peak area B) x (1 / RRF) x (Concentration B).RRF can be used for quantitative analysis of Unknown substances by means of known concentration of other substances as per the below equation,.Relative Response Factor (RRF) = Response Factor A / Response Factor B Response Factor (RF) = Peak Area / Concentration Calculation of Relative Response Factors (RRFs) A response factor (RF) is defined as the ratio between the concentration of a substance that are being analyzed and the response of the detector for that substance.Related: Theoretical plate numbers (N) and Determination of “N” in Chromatography What is a Response Factor (RF)? Estimation of Relative Response factor (RRF).
Relative Response factor (RRF) is used in different stages: Phase 1 to Phase 4 studies, in drug purity tests, Mass balance tests, in limit tests, In stability indicating methods etc.Difficulty in synthesis and isolation of Impurity Standards, for convenience and time saving.To lessen maintenance of impurity standards,.To decrease the cost on the preparation of impurity standards,.For avoid the stability issues with standards or reference standard,.Why RRF is so important in Chromatogrphay or HPLC ? The Response Factor is a relative phrase that describes the response of equal weights of one substance to that of another under the test conditions.denotes RRF as Correction factor or Response factor. The correction factor is reciprocal of the response factor (RF).The Relative detector response factor, usually referred as Response Factor (RF), represents a detector’s sensitivity for a given substance in comparison to a standard substance.RRF According to European Pharmacopoeia (Ph.Eur) USP refers RRF as Correction factor (CF) or Response factor (RF) or Relative response factor (RRF).The ratio of the responses of equal amounts of the Impurities and the drug substance is called as Relative response factor (RRF).RRF According to United States Pharmacopoeia (USP) : Different Pharmacopoeias refer the term RRF differently as described below,.RRF is calculated by the slope method with a linear range (Linearity) of Sample solutions.RRF is mainly used to correct the difference in detector response of impurities with sample (analyte) peak.RRF (Relative Response Factor) is an analytical measure used in chromatographic courses to control impurities/degradants in drug substance and drug product (Ref.What is Relative Response Factor and its importance?
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